ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。

ディテクターから出力された、電気信号を記録し、そこからピークを検出、解釈を行う。結果は、感熱紙等に印字される。装置のコントロールをしないのであれば、どのメーカーの物を使用しても問題はないが、通常は、装置のコントロールも同時に行うため、同じメーカーの物を選択する。

예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.

employs an autosampler to inject samples. In place of utilizing a syringe to thrust the sample to the sample loop, the syringe attracts sample in the sample loop.

. The working cylinder and the equilibrating cylinder for your pump on the left get solvent from reservoir A and deliver it to your mixing chamber. The pump on the best moves solvent from reservoir B on the mixing chamber.

It appears odd the far more popular method of liquid chromatography is recognized as reverse-period in lieu of regular stage. You may perhaps remember that one of the earliest examples of chromatography was Mikhail Tswett’s separation of plant pigments utilizing a polar column of calcium carbonate in addition to a nonpolar mobile phase of petroleum ether. The assignment of usual and reversed, as a result, is centered on priority.

各種の高速液体クロマトグラフィーの項目にある違いは、カラムの違いである事が多いため、装置はそのままでカラムの変更で行える場合が有る。ただし、誤って不適当な溶媒を通すとカラムを破損することがあるため、切り替えを行う際には注意が必要である。

As it makes use of a loop injection, the precision of the HPLC system generally is much better than a GC technique. HPLC isn't limited to unstable analytes, which suggests we are able to evaluate a broader array of compounds. Capillary GC columns, However, have a lot more theoretical plates, and may independent far more complicated mixtures.

4. In the event the peaks for fluoxetine and protriptyline are settled insufficiently, how may you change the cellular stage to boost their separation?

A pump forces a solvent via a column below high pressures of nearly 400 atmospheres. The column packing material or adsorbent or stationary period is often a granular materials of reliable particles such as silica or polymers.

. Solvent triangle get more info for optimizing a reversed-phase HPLC separation. The three blue circles demonstrate cellular phases consisting of an natural and organic solvent and water.

In a fuel chromatograph the pressure from the compressed fuel cylinder is ample to force the cellular stage with the column. Pushing a liquid mobile phase through a column, nevertheless, requires an excellent deal extra work, building pressures in excessive of many hundred atmospheres.

Column selection: website The stationary period inside the column interacts with analytes. Utilizing the Mistaken column chemistry may end up in inadequate resolution. Consider using another column by using a stationary phase which offers greater selectivity in your analytes.

Decreasing the quantity of acetonitrile and expanding the amount of drinking water in the mobile will raise retention situations, providing far more time for you to result a separation.